MICU1-cKO Proteomics Data

Background: Mitochondrial ATP production, essential for cardiomyocyte function, is regulated by mitochondrial Ca²⁺ (mtCa²⁺). The primary route for mtCa²⁺ influx is the mitochondrial calcium uniporter complex (mtCU). The mtCU subunit Mitochondrial Calcium Uptake 1 (MICU1) limits mtCa²⁺ uptake, preventing mtCa²⁺ overload. Although elevated mtCa²⁺ has been observed in multiple diseases including heart failure, its effects on heart function remain elusive.Methods: To investigate the impact of elevated mtCa²⁺ in adult hearts, we generated a mouse model with cardiomyocyte-specific tamoxifen-inducible <i>Micu1</i> deletion (<i>Micu1</i>^<em>cKO</em>). Cardiac function was assessed through echocardiography. Mitochondria, adult cardiomyocytes, and tissue extracts were isolated from the left ventricle (LV) and right ventricle (RV) for comprehensive analysis at multiple timepoints ranging from 1 week to 9 weeks post-tamoxifen injection.