T. gondii mRNA abundance of genes of interest (Unprocessed FPKM values).

mRNA abundance of T. gondii genes of interest in raw FPKM values. RNA samples were isolated from infected dishes paired to infected metabolomics dishes and RNAseq was performed to generate the transcriptomes. Genes throughout central metabolism are expressed at a high level throughout the parasite lytic cycle. Phosphoglycerate dehydrogenase (PHGDH), hypoxanthine phosphoribosyltransferase (HXGPRT), adenosine kinase (AK), 5-phosphoribosyl-1-pyrophosphate synthase (PRS1), hexose kinase (HK), pyruvate kinase (PK), fructose-1,6-bisphosphate aldolase (FBA), lactate dehydrogenase 1 (LDH1), aconitase (ACN), alpha-ketoglutarate dehydrogenase (A-KGDH), succinate reductase (SCR), fumarase (FUM), isocitrate dehydrogenase 2 (IDH—TCA), isocitrate dehydrogenase 1 (IDH-API), malate dehydrogenase (MDH), citrate synthase (CS), succinyl-CoA synthetase (SCS), glucose-6-phosphate dehydrogenase putative 1 (G6PD 1), glucose-6-phosphate dehydrogenase putative 2 (G6PD 2), 6-phosphogluconate dehydrogenase putative 1 (6PGD 1), 6-phosphogluconate dehydrogenase putative 2 (6PGD 2), ribose-5-phosphate isomerase (R5PI), ribulose-5-phosphate epimerase (Ru5PE), transaldolase (TA), transketolase (TK), sedoheptulose bisphosphatase (SBPASE).