Levels of the NF-��B and p50 DNA-binding, proteins and mRNAs in the PFC of alcoholics and control subjects

mRNA levels are presented as normalized relative expression levels. Protein levels are presented as relative values calculated from the optical density of p65, p50 or IKK�� bands as a percent from internal control samples and normalized to Memcode staining. Protein and mRNA levels were analyzed in 15 control subjects and 15 alcoholics. NF-��B (Act), constitutively active NF-��B; NF-��B (Tot), total NF- ��B activated by 0.6% DOC. Values for DNA binding activity are presented as percent of those obtained with the internal control samples loaded on each gel (control group: n = 11; alcoholic group: n = 13). Significance of differences between groups was evaluated by a Student's t-test, b covariance by multiple regression analysis (RELA, covariate pH), or c Mann Whitney U-test dependent on the normality of data distribution.