Text S1 - Circulating Virus Load Determines the Size of Bottlenecks in Viral Populations Progressing within a Host

Online supporting information. The online Text S1 contains additional detailed information on: i) full data sets corresponding to all results presented in this study, ii) the two statistical methods used to estimate the size of viral population bottlenecks at leaf entry, iii) the experiment showing the CaMV sap load and bottleneck size estimates on a single set of plants, iv) the Q-PCR conditions and primers, v) the detailed method for stylectomy and the problems encountered, and finally vi) the estimates of the CaMV load in aphids fed on artificial Parafilm membranes.
(DOC)