Caenorhabditis briggsae Mitochondrial Genome Assemblies of CaeNDR Whole-Genome Sequencing Data
收藏Figshare2025-06-02 更新2026-04-28 收录
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https://figshare.com/articles/dataset/_i_Caenorhabditis_briggsae_i_Mitochondrial_Genome_Assemblies_from_CaeNDR_Whole-Genome_Sequencing_Data/29208974
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This dataset contains 1927 mitochondrial genome assemblies for Caenorhabditis briggsae, generated from whole-genome sequencing data (C. briggsae releases 20250626) downloaded from the CaeNDR. The dataset include:Genome sequences (FASTA format)Assembly graph (GFA format)Annotation (GFF3 format)CDS (FFN format)Protein sequences (FAA format)The data downloaded from CaeNDR consisted of quality-controlled and aligned whole genome reads. Reads aligned to the mitochondrial genome were extracted and subjected to an initial assembly using SPAdes (v4.0.0) with a k-mer range of 127, 117, 107, 97, 87, and 77. If subsequent annotation revealed anomalies—such as abnormal gene lengths, gene counts, or gene orders—the assembly graph was manually inspected. Based on this inspection, the mitochondrial genome was either resolved manually, reassembled using Unicycler (v0.5.1) or NOVOPlasty (v4.3.5), or deemed unassemblable. The CaeNDR 20250626 release includes 2,022 strains, of which 1,927 are successfully assembled and annotated, while 95 failed assembly (63 due to the presence of complex NCR4, 2 due to insufficient sequencing depth and 30 identified as mixed strains)
创建时间:
2025-06-02



