Additional file 5 of DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biology

Additional file 5: Table S2. Cost for generating DIMPLE libraries. Listed are the reagents used in generating the Kir2.1 library, the number of these reagents used per sublibrary, their estimated cost based on list prices (not-institutionally agreed discounts) as of July 26 2022, the number of reactions for a 9 sublibrary gene 436 amino acids long such as Kir2.1, and total cost for that reagent across the library. Below is listed the total estimated cost for Kir2.1, $3235.34, and cost per variant, $0.28. Both are likely largely over-estimated for academics due to using list prices. Compared to nicking mutagenesis (0.22$/variant in 2016 excluding QC sequencing) is far more expensive than a comparable gene ($0.15/variant) using DIMPLE.