Drosophila attack inhibits hyphal regeneration and defense mechanisms activation for the fungus Trichoderma atroviride

Fungi as animals and plants conserves the ability to respond to stressful environmental conditions as biotic an abiotic stress. A correct injury response in Eukaryotes including fungi is carried out thought the activation of signaling pathways Ca2+ related dependent of DAMPs/MAPMPs recognizing for the activation of the innate immune system. During herbivory, plants, in addition to activating pathways related to injury, activates specific responses to combat its predator. Using a transcriptional approach, here, we reported the capacity of the filamentous fungus T. atroviride to activate or not a specific response to injury and predation to different predators also, we provide evidence of how fungivory by Drosophila melanogaster larvae inhibits the reported injury response for T. atroviride, although this predation negatively affects the hyphal regeneration thought the larval salivary compounds. Also, the activation of a putative alternate injury reproduction pathway is proposed. This paper presents the analysis of 42 RNA-seq libraries of T. atroviride mycelia under three different conditions (injury, Fungivory by D. melanogaster larvae and Fungivory by the collembola Orthonychiurus folsomi) at four time points, early times (30 and 90 minutes after treatment or insect interaction) and late times (4 and 8 h after treatment or insect interaction). Fungi mycelia at 30 min and 8h of the experiment without any stimuli (only oxygen exposed), were taken as control condition. In total three independent biological replicates ( each was a pool of three independent technical replicates) of each time point per condition were sequenced (42 RNA-seq libraries).