iPSC to endoderm differentiation experiments from the HipSci project

"Cultures of induced pluripotent stem cells (iPSC) from the HipSci project were differentiated to the endoderm lineage. The iPSC lines were differentiated i) independently as separate cultures, ii) as a co-culture comprising a mixture of cell lines. At various time points, live cells were FACS sorted from these bulk cultures, plated, and frozen. Each sorted cell taken from the mixed co-culture was later identified by its genetics using RNA sequencing."