Purification of NTS1.

Data are given ± SE. The volume of the NTFT is larger than that of the NiE because of the dilution applied to reduce the NaCl and imidazole concentrations. Recovery data of the NiFT and NiE relate to SN, whereas recovery data of the NTFT and NTE relate to the NiE loaded onto the NT-column. A theoretical value for specific binding of 10363 pmol/mg is calculated for NTS1 (molecular mass of 96.5 kDa) assuming one ligand binding site per receptor molecule. Abbreviations: SN, supernatant after ultracentrifugation; NiFT, flow-through of Ni-NTA column; NiE, Ni-NTA column eluate; NTFT, flow-through of NT column; NTE, NT column eluate; ND, not determined.