Theoretical Studies and Design Strategies of Highly Efficient Two-Photon Excited Fluorescent Probes for Hydrogen Sulfide Detection through Simulation of Excited-State Dynamics

Hydrogen sulfide (H2S) plays a critical role in numerous physiological and pathological processes, but an abnormal level of H2S in living systems can cause various diseases. To detect the level of endogenous H2S in a complicated biological system, the luminous mechanism of “turn-on” probe for H2S monitoring has been deeply explored through the simulation of excited-state dynamic processes, and the effect of different geometric modifications on optical properties has been minutely investigated based on molecular modeling. TD-DFT calculations demonstrate that line-type π-expanding in the molecular skeleton is beneficial for improving two-photon absorption (TPA) ability, but it can give rise to extremely large geometric relaxation, going against fluorescence emission. It is an effective way to suppress molecular skeleton scissoring vibration by introducing strong electron-withdrawing substituent groups (F, Cl, Br, CN) in benzopyran, and these compounds also have superior TPA properties in NIR. One of the potential materials in the application of biological imaging and H2S detection has been obtained, which simultaneously possesses easily distinguished spectra (with a Stokes shift as large as 77 nm), high luminous efficiency (with a quantum yield up to 20.07%), and large TPA cross section (952 GM at 950 nm).