Intra-tumoral microbial community profiling and associated metabolites alterations of TNBC

Triple-negative breast cancer (TNBC) presents significant challenges to female
health owing to the lack of therapeutic targets and its poor prognosis. In recent
years, in the field of molecular pathology, there has been a growing focus on the
role of intra-tumoral microbial communities and metabolic alterations in tumor
cells. However, the precise mechanism through which microbiota and their
metabolites influence TNBC remains unclear and warrants further investigation.
In this study, we analyzed the microbial community composition in various
subtypes of breast cancer through 16S rRNA MiSeq sequencing of formalin-fixed,
paraffin-embedded (FFPE) tissue samples. Notably, Turicibacter, a microbe
associated with cancer response, exhibited a significantly higher abundance in
TNBC. Similarly, mass spectrometry-based metabolomic analysis revealed
substantial differences in specific metabolites, such as nutriacholic,
pregnanetriol, and cortol. Furthermore, we observed significant correlations
between the intra-tumoral