using myocardial infarction (MI) model based on miniature pigs, 84 miRNAs were identified as the differentially expressed miRNAs between MI and control group

6 young male Juema minipigs weighting 20-25 kg were used to create MI model according to previous studies. Briefly, these pigs were raised in Guangdong Provincial Key Laboratory for Laboratory Animals, and this laboratory has been identified and recognized by Association for Assessment and Accreditation of Laboratory Animal Care International. These anesthetized pigs were randomly divided into sham operation control group (n=3) and MI group (n=3). After supine bound, these pigs were transected 7-10 cm in the left third intercostal space to expose the heart. Three MI pigs were created by permanent ligation of the trunk near one third of the apex after the first branch. The thoracic cavity was opened, and sutures were placed in the approximate position without ligation for the other three pigs of sham operation control group. BeneViewT5 and EDAN H100 were used to monitor the basic vital signs of animals. The success of ligation was judged and elevated by ST segment of electrocardiogram. After four weeks following the surgery, the myocardial infarcted areas of MI group and the corresponding areas of control group were collected and stored into liquid nitrogen soon for further suing.