Expression Of Nsd3Short-3Xflag In Multiple Cell Lines
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<strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong> <strong>Exp018 Objective:</strong>To generate cell lines stably expressing NSD3-Short-3xFLAG in relevant cell backgrounds. These cell lines can be used to assay phenotypes associated with over-expression and to study NSD3-short protein-protein / protein-chromatin interactions. The lentiviral vectors used here have been modified by replacing an IRES bicistronic element with a T2A self-cleaving peptide sequence (cloning described in exp-016) for expression of NSD3short-3xFLAG and a puromycin selection marker.
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2018-03-14
