Study on Molecular Mechanism of ZmRPN10 Regulating Leaf Angle in Maize

biquitin/proteasome-mediated proteolysis (UPP) plays a crucial role in almost all aspects of plant growth and development, proteasome subunit RPN10 mediates ubiquitination substrate recognition in the UPP process. The recognition pathway of ubiquitinated UPP substrate is different in different species, which indicates that the mechanism and function of RPN10 are different in different species. However, the homologous ZmRPN10 in maize has not been studied. In this study, the changing of leaf angle, gene expression in leaves in maize B73 and rpn10 under different brassinosteroids (BRs) concentrations were investigated. The regulation effect of BR on the leaf angle of rpn10 was significantly stronger than that of B73. Among the differentially expressed genes, CRE1, A-ARR and SnRK2 were significantly up-regulated, and PP2C, BRI1 AUX/IAA, JAZ and MYC2 were significantly down regulated. This study revealed the regulation mechanism of ZmRPN10 on maize leaf angle, and provided a promising gene resource for maize breeding.