The Bromodomain and extraterminal (BET) inhibitor attenuates inflammatory response and cell migration in human microglia

We investigated the mechanisms underlying the anti-inflammatory and anti-migratory function and genes targeted by JQ1 in lipopolysaccharide (LPS)-activated human microglia HMC3 cells using RNA sequencing. The BET inhibitor JQ1 attenuates the LPS-induced inflammatory and migratory response. In addition, LPS-induced IRF1 was inhibited by JQ1, and IRF1 suppressed microglial migration by regulating inflammation and migration genes. Overall design: The gene expression profile was performed with control HMC3 (Con), JQ1-treated HMC3 (JQ1), LPS-treated HMC3 (LPS), LPS+JQ1-treated HMC3 (LPS+JQ1). Samples were generated by deep sequencing, in triplicate, using Illumina 2500.