Molecular phenotyping of antisense oligonucleotides (ASOs) in immortalized proximal tubule epithelial cells (PTEC-TERT1)

Molecular phenotyping quantifies expression of pathway reproter genes by next-generation sequencing of pre-defined amplicons. We applied the technology to quantify pathway-level impact of ASOs to PTECs in the presence and absence of exogeneous EGF. Overall design: Five ASOs, each in three concentrations, and vehicle controls were added to the cell culture of PTEC-TERT1 cells either in the presence or in the absence of exogeneous EGF. Molecular phenotyping was performed 6 hours later. Three biological replicates were performed for each condition