Expression data from the adipose-derived mesenchymal stem cell (ASCs) line

We investigated the differentiation of adipose-derived stem cell line (ASCs) into keratinocyte-like cells in the presence of the competitive Rab7 inhibitor, CID-1067700 We utilized microarrays to pinpoint distinct categories of genes that are either up-regulated or down-regulated, and to clarify the regulatory pathways affected by Rab7 inhibition in ASCs. We differentiated ASCs into KLCs and subsequently with the Rab7 inhibitor, CID-1067700, at a concentration of 40 μM. RNA was extracted and hybridized using Affymetrix microarrays (Clariom S Human). The analysis compared three groups: CID-treated ASCs, differentiation media (DM)-treated ASCs, and vehicle (DMSO)-treated ASCs. Each group had three biological replicates