aCGH Trypanosoma cruzi G vs D11.

Comparative genomic analysis of Trypanosoma cruzi G with Trypanosoma cruzi D11. The 8x60K array was designed and produced by Agilent (Agilent Technologies, Santa Clara, CA,US) based on the T. cruzi clone CLB genome, release 9, available in TriTrypDB. Two groups of probes totaling 48,787 probes covering all the in silico chromosomes were selected. The first group of probes covered all the chromosomes, including coding and non-coding regions, with an average of one probe every 1.2 kb to give a total of 44,860 probes. To design the second group, we selected 422 specific chromosome markers (i.e., single copy genes present only in the homologous chromosomes). In order to obtain a higher coverage with these markers, probe density was increased to one probe every 120 bp.