RNA-seq of Arabidopsis thaliana roots exposed to the MAMP flg22 in the presence of a community of commensal bacteria [RST]

This study evaluates the transcriptome of Arabidopsis thaliana roots exposed to the MAMP flg22 in the presence of a 35-member bacterial SynCom Arabidopsis seeds (wild-type Col-0) were germinated on Johnson medium and grown for 7 days in the vertical position. Plants were then transferred to media containing 1 uM flg22 or not in the presence of SynCom35 (10E5 c.f.u / mL per strain), heat-killed SynCom35 or no bacteria. Plants were grown in a growth chamber in a 15-h dark/9-h light regime (21°C day /18°C night). Roots were harvested one or twelve days after treatment. The experiment includes nine biological replicates from three independent experiments.