ENGINEERING AND OPTIMIZATION OF IMPROVED SYSTEMS， METHODS AND ENZYME COMPOSITIONS FOR SEQUENCE MANIPULATION
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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PROBLEM TO BE SOLVED: To provide alternative and robust systems and techniques for versatile sequence targeting. SOLUTION: Provided is a non-naturally occurring or engineered composition comprising (A) a first regulatory element operably linked to a CRISPR-Cas based chimeric RNA polynucleotide sequence and a second regulatory element operably linked to an enzyme coding sequence encoding a CRISPR enzyme comprising at least one or more nuclear localization sequences， or (B) a guide sequence that can hybridize to a target sequence in an eukaryotic cell， and a first regulatory element operably linked to at least one or more tracr mate sequences， a second regulatory element operably linked to the sequence encoding the CRISPR enzyme， and a third regulatory element operably linked to a tracr sequence， each element being on the same or a different vector， the CRISPR enzyme being derived from a microorganism of a particular genus. SELECTED DRAWING: None
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国家林业和草原科学数据中心
创建时间：
2023-02-12
