Free hemoglobin determination atpatients’ bedside to evaluate hemolysis: supplementary data

Background: The authors report the relevance of using a point of care test (Helge R ? ) for free hemoglobindetermination and concordance of the values the with Cobas R ? 8000 and spectrophotometer methods.Results: The within-run of the point of care test was <3%. Good correlations among the threemethods were observed and an acceptable concordance for hemolysis index values from 50 mg/dl.An excellent agreement between the Cobas 8000 and the spectrophotometer was found. Conclusion:Automated methods represent methods of choice for free hemoglobin determination. An advantageof the Helge system is that it can be applied to samples experiencing a delay in evaluation due to thelong distance between the collection site and the central laboratory. Another advantage is its use at thebedside, in the monitoring of extracorporeal membrane oxygenation patients.