Experimental results of a Lateral Flow Immunochromatographic Strip Test method for rapid detection of Oseltamivir Phosphate in egg and chicken meat

This dataset contains experimental results data of a Lateral Flow Immunochromatographic Strip Test (LFIST) method for rapid detection of Oseltamivir Phosphate (OP) in egg and chicken meat.The related study investigates the efficacy of LFIST as an alternative method to High Performance Liquid Chromatography (HPLC) and Liquid Chromatography Mass Spetography (LC-MS), in detection of OP residues in poultry products. OP is an ethyl ester prodrug used to treat and prevent infections from influenza A and B viruses. OP residues can build up in humans from the consumption of affected meat, and cause gastrointestinal disturbances, abnormal neuropsychiatric symptoms and sudden death. In China its use as a veterinary drug has been banned in livestock and poultry, but remains in use illegally, particularly in chicken farming. LFIST can be used to detect OP residues, utilising the specific interaction between antibody and antigen in a sandwich format (or competitive format). The method presents advantages over HPLC and LC-MS, which are dependent on expensive analytical instruments, complex pre-treatment steps, skilled professionals and are not suitable for high-throughput assay or real-time on-site detection.These data files contain the experimental results of LFIST using colloidal gold-labelled monoclonal antibodies (mAbs) as the probe. LFIST was evaluated in term of sensitivity, specificity, and accuracy by testing OP-spiked egg samples and chicken samples. All data are in tabular .xslx spreadsheedt format, accessible via MS Excel and open office software.Table1 Titer of polyclonal antiserum against OP.xlsx - contains the determination of titres of mice sera against OP for mice 1 to 5, as well as the blank and negative control samples.Table 2 Sensitivity of polyclonal antiserum against OP.xlsx - contains the sensitivity values of mice sera against OP, for mice 1 to 5 at OP standard concentrations of 0, 1, 2, 4, 8 and 16 ng/mL.Table 3 The indirect ELISA titer of OP mAb.xlsx - contains values for OD450 nm, titre of supernatant and titre of ascites for each mAb hybridoma line reported in the related manuscript: 1D7, 2B3, 2E6 and 4G5Table 4 sensitivity of mAb against OP.xlsxcontains values of inhibitive titres of mAb against OP for each mAb hybridoma line reported in the related manuscript: 1D7, 2B3, 2E6 and 4G5 at OP standard concentrations of 0, 1, 2, 4, 8 and 16 ng/mL.Table 5 Recovery and intra-assay and inter-assay precision of the test strips for OP spiked.xlsx- contains results of accuracy test for LFIST: chicken and egg samples containing 5, 10, and 20 μg/kg of OP were tested using the same batch of strips (n=6). For inter-assay precision, three batches of strips were used in LFIST. Both inter-assay and intra-assay results are reported.Table 6 Comparison of the strip test with HPLC for three levels of OP residues in sample.xlsx- contains comparative results of the LFIST and HPLC methods for detection of OP in both chicken and egg samples at OP concentrations of 3.5, 9.7, and 27.9 μg/kg. Results from two methods were compared to the given concentrations by one-sample T test respectively and analyzed by independent-sample T test.