Additional file 1: of Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy

Figure S1. Exosome characterization by TEM and Western blot. TEM images of exosomes derived from BC cell lines demonstrated that our methodology was successful in isolating exosomes, observing double-membrane vesicles with a diameter of ~ 150 nm (A). Furthermore, Western blot characterization showed positive expression of Hsp70 and CD9 exosomal proteins in these exosomes but negative expression of GM-130, which was present in the MCF-7 lysate positive control (B). Figure S2. ROC Curve of MBC identification by EmiR-21 (Ext1). Gray line represents EmiR-21 (Ext1) values for sensibility and specificity while black dotted line represents random predictor baseline. Light gray area represents EmiR-21 (Ext1) area under the curve (AUC) = 0.777. Table S1. Univariate logistic binary regression for MBC identification. Abbreviations: CA19.9, Carbohydrate Antigen 19.9; CEA, Carcinoembryonic Antigen; Ext1, basal extraction; HR, Hazard Ratio. Table S2. Association between CTC presence and clinicopathological features. Abbreviations: CTCs, circulating tumor cells; Ext1, basal extraction; Ext2, extraction during neoadjuvant treatment; T, tumor size; N, lymph node status. (ZIP 352 kb)