Evaluation of the antibacterial activity and mechanism of action of CPF-MW1

The data provided represent the results of the titled study above. The study aimed to evaluate the antimicrobial activity and the mechanism of action of the frog-derived antimicrobial peptide CPF-MW1 against Gram-negative bacteria using E. coli ATCC 700928 as a model organism. The objectives of the study included the following:
Antibacterial activity determination (both anti-planktonic and anti-biofilm)Peptide cytotoxicity evaluation both on human erythrocytes and HaCaT cell line.Mechanism of action evaluation including time-kill kinetics, membrane permeabilisation and scanning electron microscopy.Peptide stability in physiological conditions evaluated by pre-exposure to proteolytic enzyme (trypsin) and fetal calf serum.

All data provided represents a set of three independent experiments performed in triplicate, unless stated otherwise. Data was analyzed and processed using GraphPad prism 8 software. Each data point was calculated as an average of three biological repeats each consisting of three technical repeats generating 9 data points unless stated otherwise. All data was expressed as means ± standard error of the means (SEM). The normality of data was first determined and depending on the normality of the data, multiple comparisons were tested using either the One-way ANOVA with Turkey’s post hoc tests, or the Kruskal-Wallis ANOVA with Duns’ post hoc tests. Significance was determined at a 95% confidence interval, and any p-value < 0.05 was identified as significant.

所提供的数据系上述标题研究所得结果。该研究旨在通过以大肠杆菌ATCC 700928作为模型生物，评估青蛙来源的抗菌肽CPF-MW1对革兰氏阴性菌的抗菌活性及其作用机制。研究目标包括以下内容：
抗菌活性测定（包括抗浮游和抗生物膜活性），对人类红细胞和HaCaT细胞系进行肽的细胞毒性评估。作用机制评估包括时间-杀伤动力学、膜通透性及扫描电子显微镜检查。通过预先暴露于蛋白酶（胰蛋白酶）和胎牛血清来评估肽在生理条件下的稳定性。
所提供的数据代表三次独立实验的结果，除非另有说明。数据使用GraphPad prism 8软件进行分析和处理。每个数据点均计算为三次生物重复的平均值，每次生物重复由三次技术重复组成，生成9个数据点，除非另有说明。所有数据均以平均值±标准误（SEM）的形式表示。首先确定数据的正态性，并根据数据的正态性，使用单因素方差分析（One-way ANOVA）及Turkey事后检验，或Kruskal-Wallis方差分析及Duns事后检验进行多重比较。显著性在95%置信区间内确定，任何p值小于0.05均被视为具有统计学意义。