Leptin-induced relaxation of phenylephrine-preconstricted mesenteric artery rings in different experimental groups.
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NO-dependent vasorelaxation was measured in the presence of apamin (5 µM), TRAM-34 (1 µM) and indomethacin (10 µM), and EDHF-dependent vasorelaxation was examined in the presence of L-NAME (100 µM) plus indomethacin (10 µM). Maximal relaxation (Rmax) was calculated as the leptin-induced percent decrease in tension developed in response to phenylephrine. EC50– leptin concentration (ng/ml) which induced a half-maximal relaxation of PE-preconstricted segments. Rmax and EC50 values were calculated for each individual vascular preparation and data presented in the table are mean ± SD from 6 animals per group.
*p<0.05,
**p<0.01,
***p<0.001 vs. control group (ANOVA and Tukey test).
创建时间：
2014-01-24
