Gene profile of human CD14+ monocytes and monocyte-derived macrophages

Comparison of the transcriptome of CD14+ human monocytes and CD14+ human monocyte-derived macrophages generated in the presence of M-CSF (M-MØ) or GM-CSF (GM-MØ). Overall design: mRNA profiles of human CD14+ monocytes isolated by immunomagnetic procedures from peripheral blood, and of monocyte-derived macrophages generated by culturing monocytes for 7 days in the presence of M-CSF (10 ng/ml) to generate M-MØ, or in the presence of GM-CSF (1000 U/ml) to generate GM-MØ.