Autophagy-mediated metabolic effects of aspirin

Aspirin caused a transient reduction of general protein acetylation in blood leukocytes, accompanied by the induction of autophagy. These effects on global protein acetylation could not be attributed to the mere inhibition of EP300, but most likely through the inhibition of other acetyltransferases. To investigate this possibility, we performed a quantitative label-free enriched acetylome analysis, search-ing for proteins that are deacetylated upon EP300 knockout and/or salicylate treatment.