five

Human cancer cell MCF-7 IP-PTEN MS

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NIAID Data Ecosystem2026-03-13 收录
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https://www.omicsdi.org/dataset/pride/PXD021544
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The MCF-7 cells were lysed using RIPA lysis buffer with a high salinity content to break out non-covalent binding of proteins. Then IP under partially denaturing conditions was performed and demonstrated that anti-PTEN precipitates were detected as smear bands, then mass spectrometry analysis of the smear bands was performed.
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2022-04-04
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