Expression data from Th17 cells treated with Tinospora Cordifolia

Tinospora Cordifolia, an ayurvedic herbal compound, is studied to be an effective drug in Autoimmine disease. Several in vivo mouse model systems are reported to show its efficiency as a drug of therapeutic potential to treat autoimmune diseases. Th17 cells are the major T cells involved in the inflammatory responses in autoimmune diseases. The mechanistic insight into the therapeutic efficiency of Tinospora cordifolia is yet not studied. In this study we have tried to unravel the mechanism of action for the effect of water extract of Tinospora cordifolia on Th17 cells using genome wide expression approach. Naïve CD4+CD25- T cells were polarized to Th17 cells and were treated with 1500, 1000 and 500 µg/ml of Tinospora Cordifolia water extract (AETC). Untreated samples were used as a control. On day 4, cells were harvested and total RNA was isolated followed by hybridization on Affymetrix Gene Chip MTA 1.0 array according to manufacturer’s instruction. Gene expression analysis was performed using Transcriptome Analysis Console (TAC) and functional analysis done by using the Enrichr tool.