Regulation of circular RNA circNFATc3 in cancer cells alters proliferation, migration and oxidative phosphorylation

Regulation of circular RNA circNFATc3 in cancer cells alters proliferation, migration, and oxidative phosphorylation.NFATC3 gene (which is formed from back splicing of exon 2 and 3) in solid tumors was identified from transcriptomic data. Here we show that circNFATC3 loss of function experiments using RNAi mediated circRNA silencing and differential expression using RNAseq for identifying molecular phenotypes. We used MDA-MB-231 cells for RNA sequencing, two different siRNA constructs were used for RNA seq along with the scrambled control. RNA seq was performed Illumina platform, the data analysis was done wit PARTEK genomic suite.