DENN-4, the putative RAB-10 GEF, is required for dendritic morphogenesis in PVD.

(A) Schematic of somatic CRISPR targeting construct used to produce conditional denn-4 mutations. (B) Representative image of PVD::GFP in a heat-shocked WT control animal. (C) Representative image of PVD::GFP in the conditional denn-4 CRISPR allele. (D) Efficiency of conditional CRISPR denn-4 knockout in generating PVD-phenotype in all conditions and genotypes tested. (E) Quantification of subcellular distribution of branch complexity in this allele. Scale bars represent 20 μm. Error bars represent SEM. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by 2-way ANOVA with Tukey’s multiple comparisons test. N≥3 for all genotypes.