Spatial Multimodal Analysis: MALDI-MSI and Spatial Transcriptomics within the same tissue section

We present a spatial omics approach that merges and expands the capabilities of independently performedin situassays on a single tissue section. Our spatial multimodal analysis combines histology, mass spectrometry imaging, and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and low-molecular weight metabolites across tissue regions. We demonstrate the potential of our method using murine and human brain samples in the context of dopamine and Parkinson's disease. Overall design: Tissue sections processed with MALDI-MSI were eventually analyzed using Visium. Controls for some of the samples are included. *** From Submitter: We are restricted to deposit raw sequencing from humans to SRA. Those will be deposited on the Swedish federated EGA node and we will link when it became available to us. ***