Draft Genome Sequence of a mcr-1/ IncI2-carrying multidrug-resistant Escherichia coli B1: ST101 isolated from meat and meat products in Egypt

The aim of this study was to investigate the occurrence of the plasmid mediated colistin resistance among gram negative bacteria isolated from meat and meat products in Egypt and to report the draft genome sequence of a mcr-1/ IncI2-carrying multidrug-resistant E. coli B1: ST101. We reported a mcr-1-positive multidrug-resistant E. coli strain isolated from sausages in Egypt. The genome size of E. coli strain was calculated at 5,044,715 bp, comprising a total of 226 contigs, with a G+C content of 50.5%. The E. coli strain belonged to sequence type ST101 (Phylogroup B1). The mcr-1 gene was located on an IncI2 type self-conjugative plasmid of size 64.6 kb. This strain showed MDR phenotype with a colistin MIC of 4 mg/L. It mediates resistance to colistin (mcr.1), beta-lactams (blaTEM-1), phenicol (floR), trimethoprim (dfrA12), aminoglycoside resistance (aac(3)-IIa, aph(3'')-Ib and aadA2), macrolide (mph(A) and mdf (A)), tetracycline (tet(A) sulfonamide (Sul1 and Sul2), quinolones (qnrS1) and gyrA mutation p.S83L (Nalidixic acid and Ciprofloxacin). Here we reported first draft genome sequence of a mcr-1/ IncI2-carrying multidrug-resistant Escherichia coli B1: ST101 isolated from meat and meat products in Egypt. This study highlights the potential role played by meat and meat products in the spread of colistin resistance to human. This draft genome sequence can provide valuable information for better understanding of the dissemination of MDR enteric bacteria in food environments.