Genome wide ChIP-seq analysis of human TOP2B occupancy in KG-1 myeloid cell line

TOP2B is involved in transcriptional initiation in response to nuclear hormone ligands and plays a role in transcriptional elongation. Whole genome TOP2B ChIP-seq was carried out on human KG-1 cells in the presence and absence of the topoisomerase inhibitors mitoxantone and etoposide. Chromatin was assayed for TOP2B occupancy by peak calling with SISSRs and MACS. Whole genome TOP2B ChIP-seq was carried out on human KG-1 cells in the presence (60 mins exposure) and absence of the topoisomerase inhibitors etoposide and mitoxantrone. "Input" control samples were also sequenced for background detection and comparison.