Single-cell RNA-seq of mouse cells from the dorsal cerebral cortex during its formation

Dorsal cortices from embryonic day (E10.5) to postnatal day 3 (P3) were micro-dissected and dissociated into single cell suspensions using Papain and Ovo-mucoid mix. Cells were washed with L15 medium and FAC-sorted for GFP positive NSCs using FACSariaIII (BD Biosciences) derived from Hes5::GFP transgenic embryos for NSCs and Tbr2::GFP transgenic embryos for BPs and NBNs. 1094 cells, either neural stem cells, basal progenitors and newborn neurons, daily from embryonic day 10.5 to third post-natal day.