ct of PROTAC #89, which targets an endogenously SNAP-tagged clathrin light chain, on the HAP1 cell proteome

We would like to investigate what effect degradation of the clathrin light chain has on the HAP1 proteome. We therefore tagged the clathrin light chain (CLC) in HAP1 cells endogenously with a SNAP-tag via CRISPaint. The degradation of the SNAP-tagged protein is induced by the addition of a SNAP-PROTAC (PROTAC #89). The proteomes of a short (6 h) and long (24 h) treatment with PROTAC #89 are compared against the corresponding DMSO controls using LFQ.