RNA profiling of IL-34 overexpressing acute myeloid leukemia (AML) cells

Acute myeloid leukemia (AML) is a highly heterogeneous disease. In our study, we found that overexpressing IL-34 in AML cells accelerates the AML progression and shorten the mice survival, which is medicated by LSC level. We used one of the LSC markers, c-Kit, to divide AML cells. The goals of this study aim to reveal the mechanism mediated by IL-34 overexpression, we performed the RNAseq profiling of MA9-c-kit-, MA9-c-kit+ and MA9-IL-34 AML cells. c-kit+ cells were isolated from MA9 and MA9-IL-34 mice. c-kit- cells were isolated from MA9 mice. mRNA profiles of these cells were generated by RNA sequencing.