METHOD FOR RAPIDLY AND EFFICIENTLY CREATING DIRECTED GENE MUTATED NON-TRANSGENIC PLANTS AND ITS APPLICATIONS
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
The invention relates to a plant genetic engineering field， and more particularly to a method for creating directed gene mutated non-transgenic plants. The method including performing a transgenic method onto directed gene mutated plants by introducing exogenous nucleic acid molecules; wherein the transgenic method includes introducing constructs into the directed gene mutated plants， each of the constructs contains a first nucleic acid molecule and a second nucleic acid molecule， wherein the first nucleic acid molecule serves as a gene editing element， and the second nucleic acid molecule serves as a lethal or stop development element， and can be used in a plant gene editing system such as CRISPR/CAS9. It can actively and automatically eliminate plant transgenic fragments， leaving enough time for gene editing elements to perform directed gene editing before removing transgenic fragments， providing a simple and effective method for gene editing without transgenic plants.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
