PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent - Supplementary dataset

Aim: To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA
polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents.
Methods: A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyICPBNPs
were stable and monodisperse, maintained the native absorbance properties of both polyIC and
PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies.
Results: PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of
HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free
polyIC in CD4 and CD8 T cells. Conclusion: PBNPs function as efficient carriers of nucleic acids to directly
reverse HIV latency and enhance immune activation.