Chromatin accessibility and transcriptome landscapes of Lactobacillus reuteri inhibit Escherichia coli and Salmonella in porcine intestinal epithelial cells

L. reuteri is a probiotic with bacteriostasis, which can effectively inhibit the activity of pathogens, and has the potential to act as a feed additive to regulate intestinal microecological balance. We used ATAC-seq and RNA-seq methods to globally delineate the biological processes and accessible chromatin regions changes in intestinal epithelial cells stimulated by E. coli and Salmonella, as well as the epigenetic regulatory mechanisms by which L. reuteri inhibits pathogens. Totally, we provided 15 ATAC-seq and 15 RNA-seq datasets and demonstrated the quality of the datasets by quality control and reproducibility analysis, in which we found some interested genes and accessible chromatin regions. In conclusion, our data set share new insights into the expression pattern of functional genes after stimulation of porcine intestinal epithelial cells by pathogenic bacteria leading to diarrhea, and provide new ideas for the epigenetic regulatory mechanism of bacteriostatic effect of L. reuteri, providing a valuable resource for epigenomic studies of livestock and poultry diseases.