File S1 - Increase in Net Activity of Serine Proteinases but Not Gelatinases after Local Endotoxin Exposure in the Peripheral Airways of Healthy Subjects

Table S1. Symptom assessment after the first bronchoscopy (B.I) in 18 healthy subjects. n =  number of subjects reporting each symptom. Table S2. Blood concentrations of C-reactive protein (CRP), total leukocytes (LPC) and polymorphonuclear leukocytes (PMN's) at baseline prior to bronchoscopy I (B.I), and 24 or 48 hours (h) after intra-bronchial endotoxin exposure in healthy subjects. Table S3. Recovery of and cell viability in bronchoalveolar lavage (BAL) fluid samples harvested after intra-bronchial exposure to vehicle (Veh) and endotoxin (E), respectively, in 18 healthy subjects. Samples were harvested either 24 (n = 11) or 48 (n = 7) hours after the exposure. Figure S1. Photo of representative zymography gel, based upon analysis of cell-free bronchoalveolar lavage (BAL) fluid samples harvested after intra-bronchial exposure to vehicle (no arrow) and endotoxin (arrow), respectively, at 24 hours (h, black arrow) or 48 h (open arrow) in healthy subjects. The samples display three bands representing different molecular weights, corresponding to matrix metalloproteinase (MMP)-2 (72 kD), MMP-9 (92 kD) and a complex bound form of MMP-9 (130 kD). *Two lanes from the same subject. Figure S2. Photomicrograph of representative cytospin slides showing bronchoalveolar lavage (BAL) cells containing intracellular matrix metalloproteinase (MMP)-9 harvested after intra-bronchial exposure to endotoxin and vehicle, respectively, in healthy subjects. Immunoreactivity to MMP-9 is red and polymorphonuclear (PMN) cells with positive staining are indicated with arrows. Panels show samples from segments exposed to a) endotoxin and b) vehicle from the same subject at 24 hours (h) and c) endotoxin at 48 h after exposure from another subject. In panel c, the PMN indicated by an arrow is engulphed by a macrophage.
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