Race-specific alterations in DNA methylation among middle-aged African Americans and Whites with Metabolic Syndrome

Metabolic syndrome (MetS) is a cluster of cardiometabolic risk factors for all-cause mortality, cardiovascular disease, and cancer. Identifying epigenetic alterations associated with MetS in African Americans (AAs) and Whites may provide insight into genes that influence its differential health outcomes. We examined DNA methylation (DNAm) and performed an epigenome-wide association study (EWAS) of MetS among AA and Whites with and without MetS. We assessed age, race and poverty status associated DNAm among AAs (n=225) and White (n=233) adults using NCEP-ATP III guidelines. Genome-wide DNAm measurement was assessed using Illumina Infinium Methylation EPIC BeadChip. Differentially methylated positions (DMPs) and differentially methylated regions (DMRs) were identified using <i>dmpFinder</i> and <i>bumphunter</i>. EWAS was performed using CpGassoc. We found significant DMPs associated with age, poverty status and MetS in each race. <i>GSTT1(</i>Glutathione S-Transferase Theta 1) was one of the top hypermethylated genes and <i>MIPEP</i> (Mitochondrial Intermediate Peptidase) was one of the most hypomethylated genes when comparing AAs with and without MetS. <i>PPP1R13L</i> (Protein Phosphatase 1 Regulatory Subunit 13 Like) was the top hypermethylated and <i>SCD</i> (stearoyl-CoA desaturase-1) was one of the most hypomethylated genes for Whites with and without MetS. EWAS results showed that DNAm differences might contribute to MetS risk among Whites and AAs since different genes were identified in AAs and Whites. We replicated previously identified MetS associated genes and found that Thioredoxin-interacting protein (<i>TXN1P</i>) was statistically significantly differentially expressed only in Whites. Our results may be useful in further studies of genes underlying differences in MetS among AAs and Whites.