Celine Baldeyron, Gaston Soria, Daniele Roche, Adam J. L. Cook, Genevieve Almouzni (2012) CIL:38049, Mus musculus. CIL. Dataset

3T3 cells, 5min after the induction of DNA damage by microirradiation with a 405 laser. Cells were immunostained with antibody against H3K9me3 (green), a post-translational histone modification enriched at the pericentric heterochromatin domains. DNA damage sites (sites of irradiation) were visualized indirectly by H2AX (red) staining, DNA is stained with Hoescht 33258 (blue). This image is part of the original data set contributing to Baldeyron et al. (2011) HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair J. Cell Biol. 193: 81-95.

在微光照射405激光诱导DNA损伤后的5分钟内，对3T3细胞进行免疫染色，使用针对H3K9me3（绿色）的抗体，该翻译后修饰的组蛋白富集于着丝粒异染色质区域。通过H2AX（红色）染色间接可视化DNA损伤位点（照射点），DNA使用Hoechst 33258（蓝色）染色。此图像是Baldeyron等人（2011年）发表在《细胞生物学杂志》（J. Cell Biol.）上的HP1α通过p150CAF-1促进同源重组修复的DNA损伤招募的原始数据集之一，论文编号为193: 81-95。