2H10, bivalent 2H10 and mutant bi-2H10 IC50 titers against HIV-1 in TZM-bl cells.

Neutralization activities of 2H10, bivalent 2H10, W100A mutant bi-2H10 and as a control 2F5 were determined against the indicated viruses. The type, clade, tier and the accession code of the envelope protein sequence are given for each virus strain. The wild-type bi-2H10s are named bi-15GS and bi-17GS and have a 15 and 17 residue linker respectively. The W100A mutant bi-2H10 is named W100A bi-15GS and has a 15 residue linker. The column labeled Tier indicates whether the virus is classified for 1,2, or 3 assessment of neutralizing antibodies [116]. The column labeled Epitope sequence, shows the sequence that aligns with the part of the HBX2 sequence to which 2H10 is directed. The underlined, boldfaced residues are essential for the binding of 2H10 and are equal to the residues at the equivalent positions in HXB2, which was used for immunization. Residues in grey are at the position of an essential residue, but deviate from the HBX2 equivalent. • indicates IC50>50 µg/ml; the empty cells represent non-performed measurements.