Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth [bulkATACseq_AKPS]. Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth [bulkATACseq_AKPS]

Human engineered CRC organoids were equipped with the intestinal stem cell reporter STAR reflecting transcriptional activity of ASCL2. Bulk ATAC-sequencing was performed on STAR populations after 5 days and after 12 days of plating single STAR+ or STAR- cells. Overall design: Organoids were grown from single STAR+ or STAR- cells (APCKO/KO; KRASG12D/-; TP53KO/KO; SMAD4 KO/KO (AKPS)). After 5 days and after 12 days, organoids were harvested, dissociated to single cells, and between 75-100 k STAR+ and STAR- cells were collected in technical replicates for bulk ATAC-seq.