Dynamic transcriptome profiling of mouse spleen infected with extraintestinal pathogenic Escherichia coli

Extraintestinal pathogenic Escherichia coli (ExPEC) is a common bacterial strain causing diverse diseases in humans and animals. To analyse the detailed mechanisms underlying ExPEC-mediated sepsis in humans, the transcriptome response of mice at 3h,6h, and 12h after ExPEC infection was analyzed by RNA-seq of mouse spleen samples. Overall design: The transcriptome response of mice to intraperitoneal injection with ExPEC was analyzed by RNA-seq of mouse spleen samples. 4 groups with 3 biological replicates, 3P1-3 for the PBS-injected group at 3 hpi (designated g1), 3X1-3 for the ExPEC-infected group at 3 hpi (designated g2), 6X1-3 for the ExPEC-infected group at 6 hpi (designated g3), and 12X1-3 for the ExPEC-infected group at 12 hpi (designed g4).