RNA Sequencing of HEK293T cells expressing a Dual-specificity Aptamer that specifically increases O-GlcNAcylation on beta-catenin when EZH2 was knocked-down

O-GlcNAc is a dynamic post-translational modification on thousands of intracellular proteins, it regulates protein functions and is involved in many metabolic diseases. Using a dual-specificity aptamer, we targeted the O-GlcNAc transferase (OGT) to endogenous ß-catenin and specifically increased O-GlcNAcylation of ß-catenin. To study how O-GlcNAcylation of ß-catenin regulates the transcriptome, we performed RNA sequencing on HEK293T cells expressing individual (Ctrl.) or dual-specificity aptamers. This dataset is from the control experiment in which EZH2 was knocked-down with a shRNA in all samples. Overall design: 12 samples from 4 groups of HEK293T cells were analyzed: cells expressing Ctrl (T1 · bc339) or Dual-specificity (3JB8F+12) aptamers were exposed to Wnt- or Wnt+ medium. Each group contains 3 replicates. Cells in all replicates stably expressed a shRNA that targets EZH2.