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Whole-genome sequencing analysis of CRISPR-tolerant mutants. Escherichia coli

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NIAID Data Ecosystem2026-05-01 收录
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To investigate whether ISs transpositions into cas genes could potentially lead to concurrent transpositions into other genomic loci or induce genomic rearrangements and instability, we performed whole-genome sequencing of 11 CRISPR-tolerant mutants that survived after CRISPR/Cas-induced killing and an uninduced control strain, SY221, containing only the cas-expressing plasmid without sgRNA guides.

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2023-06-26
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