RNA-seq Analysis of CD1 background spermatogonia with NRRA treatment Transcriptomes. RNA-seq Analysis of CD1 background spermatogonia with NRRA treatment Transcriptomes

Purpose: The goals of this study are to compare CD1 background spermatogonia veh with NRRA treatment transcriptome profiling (RNA-seq) and to evaluate protocols for in vitro induced meiosis system.Methods: CD1 background spermatogonia mRNA profiles of veh and NRRA treatment were generated by deep sequencing, in duplicate. Methods: CD1 background spermatogonia veh with NRRA treatment were generated by deep sequencing, in duplicate. Conclusions: Our study represents the first detailed analysis of induced meiosis spermatogonia transcriptomes, with biologic replicates, generated by RNA-seq technology. Overall design: CD1 background spermatogonia mRNA profiles Veh and NRRA treatment were generated by deep sequencing, in duplicate, using Illumina. Veh: complete spermatogonial culture medium NRRA: nutrient restriction medium plus retinoic acid