Kinetic constants determined for SACTE_2347 variants.

aPure β-1,4 d-mannan.
bAcetylated glucomannan contain mannan (60%) and glucose (40%).
cLocust bean gum is a natural galactomannan with composition of ∼3.5 mannose per galactose.
dIL-pine has the following composition: 34% glucose; 9% xylose; 8% mannose; 4% arabinose, and 8% galactose.
SACTE_2347 did not hydrolyze cellulose, xylan and other polysaccharides described in the Materials and Methods, and likewise did not react with fluorogenic small molecule analogs.